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Oggetto:

(OPZIONALE) BIOTECNOLOGIE APPLICATE AI TRAPIANTI D’ORGANO

Oggetto:

Biotechnologies applied to organ transplants

Oggetto:

Anno accademico 2022/2023

Codice attività didattica
SME1026
Docente
Prof. Renato Romagnoli (Docente Titolare dell'insegnamento)
Corso di studio
[f007-c201] laurea spec. in biotecnologie mediche - a torino
Anno
2° anno
Periodo
Secondo semestre
Tipologia
A scelta dello studente
Crediti/Valenza
2
SSD attività didattica
MED/18 - chirurgia generale
Erogazione
Tradizionale
Lingua
Italiano
Frequenza
Obbligatoria
Tipologia esame
Orale
Oggetto:

Sommario insegnamento

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Obiettivi formativi


Il corso si propone di fornire uno scenario aggiornato sull’applicazione delle biotecnologie nei trapianti d’organo. Il corso verterà sulle applicazioni nell’ambito della cura di alcune patologie che possono portare ad un’indicazione a trapianto, della preservazione e ricondizionamento degli organi da trapiantare e della prevenzione di alcune complicanze dei trapianti d’organo. Saranno trattate sia le applicazioni già in uso clinico che quelle ancora oggetto di ricerca o in sperimentazione clinica. Particolare enfasi sarà posta nell’evidenziare ambiti di possibile ricerca e utilizzo in aree dove i bisogni clinici dei pazienti trapiantati rimango tuttora disattesi dalle terapie disponibili. L’obiettivo formativo principale sarà stimolare una riflessione su quali siano le aree di ricerca per l’impiego delle biotecnologie in trapiantologia e sui metodi con cui eventuali studi dovrebbero essere condotti.

The aim of this course is providing an up-to-date picture on the applications of
biotechnologies in the setting of organ transplantation. The course will focus on the
applications of biotechnology for the cure of diseases that may lead to organ
transplantation, organ procurement and preservation, and outcome improvement. Both
experimental and consolidated clinical approaches will be discussed. A special emphasis will
be dedicated to highlighting the setting where unattended medical needs may prompt new
directions of research. The main goal will be stimulating a reflection to identify areas where
biotechnologies may provide and answer and about the methods by which this research
should be conducted.

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Risultati dell'apprendimento attesi

Il partecipante dovrà sviluppare una conoscenza di base del percorso di un paziente sottoposto a trapianto e delle modalità pratiche con cui i trapianti vengono eseguiti.

Questo richiederà una conoscenza dei tipi di donatori, delle modalità di prelievo e di preservazione degli organi e dei risultati del trapianto stesso. In particolare, il partecipante dovrà essere in grado di identificare e discutere le aree critiche in cui un miglioramento delle pratiche attuali è necessario, prospettando, anche sulla base del proprio background, ambiti di potenziale utilizzo delle biotecnologie.

 

Expected formative goals:
The attendees are expected to develop a basic understanding of the standard solid organ
transplant pathway and of the practical modalities by which organ transplants are
performed.


This will entail acquiring knowledge about organ donor types, procurement modalities,
organ preservation, and transplant outcomes. In particular, the attendees will be asked to
be capable of identifying aspects where improvement is needed and, also based on her/his
background, prospect possible solutions.

 

 

Oggetto:

Programma

  • Overview sul trapianto di fegato:
    • Indicazioni (staminali in epatite fulminante)
    • Tipi di donatori
    • Fasi del trapianto
    • Complicanze:
      • Danno da ischemia-riperfusione,
      • Disfunzione precoce e sue implicazioni
      • Complicanze biliari
    • Risultati
  • Tecniche di prelievo e preservazione:
    • Static cold storage
    • Perfusione ex-situ ipotermica
    • Perfusione ex-situ normotermica
    • Perfusione regionale normotermica
  • Il viability assessment:
    • Perfusione regionale normotermica
    • Perfusione ex-situ ipotermica
    • Perfusione ex-situ normotermica
    • Viability epatocitaria e colangiocitaria
  • Tecniche di ricondizionamento:
    • Cellule staminali e microvescicole
    • Filtri per citochine
    • Defatting
    • Prevenzione del danno colangiocitario
  • Prospettive future
    • Trattamento dell’insufficienza epatica acuta
    • Perfusione ex-situ normotermica di lunga durata
    • Supercooling

English:
 Overview on liver transplantation
o Indications (stem cells for acute liver failure)
o Donor types
o Phases of liver transplantation
o Complications
 Ischemia-reperfusion injury
 Early allograft dysfunction and its complications
 Biliary complications
o Outcomes
 Organ procurement and preservation
o Static cold storage
o Ex-situ hypothermic machine perfusion
o Ex-situ normothermic machine perfusion
o Normothermic regional perfusion
 Viability assessment
o Normothermic regional perfusion
o Ex-situ hypothermic machine perfusion
o Ex-situ normothermic machine perfusion
o Hepatocellular and cholangiocellular viability
 Organ reconditioning
o Stem cells and derived products
o Cytokine filters
o Defatting
o Cholangiocellular injury prevention
 Future perspectives
o Treatment of acute liver failure
o Long-term ex-situ normothermic machine perfusion
o Supercooling

Oggetto:

Modalità di insegnamento

Lezioni frontali della durata complessiva di 16 ore, condotte con ausilio di materiale iconografico della Chirurgia Generale 2U e spazio per discussione di scenari clinici e di ricerca

 

Oggetto:

Modalità di verifica dell'apprendimento

Esame orale

 

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Attività di supporto

Non previste

 

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Testi consigliati e bibliografia



Oggetto:
Libro
Titolo:  
The Puzzle People
Anno pubblicazione:  
1992
Editore:  
University of Pittsburgh Press
Autore:  
Thomas E. Starzl
Obbligatorio:  
No
Oggetto:

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